Western Blot Analysis is a laboratory technique used to detect specific proteins in a sample of tissue or fluid. This technique involves three primary steps: gel electrophoresis, protein transfer, and antibody detection.
First, the sample is separated based on the size of the protein by gel electrophoresis. Second, the isolated proteins are transferred to a membrane, usually made of nitrocellulose or polyvinylidene fluoride (PVDF). This process is called protein transfer, where proteins are immobilized on a membrane, creating a replica of the original gel. Finally, an antibody specific to the protein of interest is added to the membrane. The antibody then binds to the protein and produces a signal that can be detected, usually by chemiluminescence.
Western Blot Analysis is used in the healthcare industry to diagnose various diseases and disorders such as HIV, hepatitis C, Lyme disease, and Alzheimer's disease. It is also used in research to help identify how cells function and to develop new therapies to treat diseases. Western Blot Analysis remains a popular tool in medical research and remains highly valuable in the healthcare industry due to its ability to identify specific proteins.
Western Blot Analysis
Health Care Term
Western Blot Analysis is a laboratory technique used to detect specific proteins in a sample of tissue or fluid. This technique involves three primary steps: gel electrophoresis, protein transfer, and antibody detection.
First, the sample is separated based on the size of the protein by gel electrophoresis. Second, the isolated proteins are transferred to a membrane, usually made of nitrocellulose or polyvinylidene fluoride (PVDF). This process is called protein transfer, where proteins are immobilized on a membrane, creating a replica of the original gel. Finally, an antibody specific to the protein of interest is added to the membrane. The antibody then binds to the protein and produces a signal that can be detected, usually by chemiluminescence.
Western Blot Analysis is used in the healthcare industry to diagnose various diseases and disorders such as HIV, hepatitis C, Lyme disease, and Alzheimer's disease. It is also used in research to help identify how cells function and to develop new therapies to treat diseases. Western Blot Analysis remains a popular tool in medical research and remains highly valuable in the healthcare industry due to its ability to identify specific proteins.